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在人工毛细血管上生长的细胞中生产委内瑞拉马脑炎病毒。

Production of Venezuelan equine encephalitis virus in cells grown on artificial capillaries.

作者信息

Johnson A D, Eddy G A, Gangemi J D, Ramsburg H H, Metzger J F

出版信息

Appl Environ Microbiol. 1978 Feb;35(2):431-4. doi: 10.1128/aem.35.2.431-434.1978.

DOI:10.1128/aem.35.2.431-434.1978
PMID:637540
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC242848/
Abstract

Primary cell cultures, a continuous cell line, and a diploid cell line were grown on an artificial capillary system. The cells were subsequently infected with Venezuelan equine encephalitis virus, and viral replication was studied. Extracellular fluids harvested from this system contained high titers of virus and were relatively free of cell debris.

摘要

原代细胞培养物、一个连续细胞系和一个二倍体细胞系在人工毛细管系统上生长。随后这些细胞用委内瑞拉马脑炎病毒感染,并对病毒复制进行了研究。从该系统收集的细胞外液含有高滴度的病毒且相对不含细胞碎片。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2089/242848/c2e5b031e47e/aem00217-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2089/242848/c2e5b031e47e/aem00217-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2089/242848/c2e5b031e47e/aem00217-0221-a.jpg

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Production of Venezuelan equine encephalitis virus in cells grown on artificial capillaries.在人工毛细血管上生长的细胞中生产委内瑞拉马脑炎病毒。
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本文引用的文献

1
QUANTITATIVE USE OF THE ELECTRON MICROSCOPE IN VIRUS RESEARCH. METHODS AND RECENT RESULTS OF PARTICLE COUNTING.电子显微镜在病毒研究中的定量应用。粒子计数的方法及最新结果。
Lab Invest. 1965 Jun;14:831-63.
2
Hormone production by cells grown in vitro on artificial capillaries.在人工毛细血管上体外培养的细胞的激素分泌
Exp Cell Res. 1974 Mar 15;84(1):251-4. doi: 10.1016/0014-4827(74)90403-0.
3
Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production.
In Vitro. 1973 Mar-Apr;8(5):323-32. doi: 10.1007/BF02619056.
4
Improved method for production of attenuated Venezuelan equine encephalomyelitis (TC-83 strain) vaccine.生产减毒委内瑞拉马脑炎(TC - 83株)疫苗的改进方法。
J Clin Microbiol. 1976 Apr;3(4):460-2. doi: 10.1128/jcm.3.4.460-462.1976.
5
A hybird artifical pancreas.一种混合人工胰腺。
Trans Am Soc Artif Intern Organs. 1975;21:8-15.
6
Bilirubin conjugation by an artificial liver composed of cultured cells and synthetic capillaries.
Trans Am Soc Artif Intern Organs. 1975;21:16-27.