Kozareva M, Nedkova-Bratanova N, Enikova R, Stoiano Z, Boneva M
Vutr Boles. 1981;20(3):58-63.
Bacteriological tests on feces of patients with ulcerative-hemorrhagic colitis, in exacerbation, were carried out. The same patients were dynamically followed up as regards clinical picture and paraclinical indices, rectoromanoscopy include. Quantitative and qualitative analysis of the fecal microflora was made according to the following indices: total number of mesophili aerobic microorganisms, coli-bacteria, proteus, enterococci, staphylococci, Salmonella-bacteria. The pure cultures isolated were tested according to 30 biochemical indices, their precise classification position being determined on that base. The production of the following enzymes was studied in the cultures isolated: protease, oxyreductase -- catalase and peroxidase, aminotransferase -- COT and GPT; phosphatase -- acid and alkaline, disaccharidase and urease. The data obtained from the bacteriological studies were compared with those of a control group of healthy subjects. Elevated enzyme constellation was found among the patients with exacerbated ulcerative-hemorrhagic colitis.
对处于病情加重期的溃疡性出血性结肠炎患者的粪便进行了细菌学检测。对这些患者的临床表现和包括直肠乙状结肠镜检查在内的临床旁指标进行了动态跟踪。根据以下指标对粪便微生物群进行了定量和定性分析:嗜温需氧微生物总数、大肠杆菌、变形杆菌、肠球菌、葡萄球菌、沙门氏菌。对分离出的纯培养物依据30项生化指标进行检测,并在此基础上确定其精确的分类位置。对分离出的培养物中以下酶的产生情况进行了研究:蛋白酶、氧化还原酶——过氧化氢酶和过氧化物酶、转氨酶——谷草转氨酶和谷丙转氨酶;磷酸酶——酸性和碱性、双糖酶和脲酶。将细菌学研究获得的数据与健康受试者对照组的数据进行了比较。发现在病情加重的溃疡性出血性结肠炎患者中酶谱升高。