Ochs G, Bromm B, Schwarz J R
Biochim Biophys Acta. 1981 Jul 20;645(2):243-52. doi: 10.1016/0005-2736(81)90195-4.
The inactivation of Na+ permeability in single myelinated motor nerve fibres of Rana esculenta was investigated under voltage and current clamp conditions at 20 degrees C in Ringer's solution and under blocked K+ currents. Development of inactivation and its recovery was described by two potential-dependent time constants: The smaller time constant followed the usual bell-shaped function of membrane potential, whereas the larger one was monotone-increasing with more negative potentials. Several three-state models for inactivation were investigated. The experiments could best be approximated by a model with two open and one closed state for inactivation following: open in equilibrium closed in equilibrium open. Rate constants were determined for all transitions shown from the voltage clamp experiments. The action potentials computed by means of the proposed model were in good agreement with those measured, both in Ringer's solution and under blocked K+ current conditions.
在20摄氏度的任氏液中，于电压钳和电流钳条件下，在阻断钾电流的情况下，研究了食用蛙单根有髓运动神经纤维中钠通透性的失活情况。失活的发展及其恢复由两个电位依赖性时间常数来描述：较小的时间常数遵循膜电位通常的钟形函数，而较大的时间常数则随着电位更负而单调增加。研究了几种失活的三态模型。实验结果最适合用一个具有两个开放状态和一个关闭状态的失活模型来近似：平衡时开放，平衡时关闭，开放。根据电压钳实验确定了所示所有转变的速率常数。通过所提出的模型计算的动作电位与在任氏液中和阻断钾电流条件下测量的动作电位非常吻合。
