Kress Y, Gaskin F, Brosnan C F, Levine S
Brain Res. 1981 Sep 7;220(1):139-49. doi: 10.1016/0006-8993(81)90217-1.
To test for in vivo zinc neurotoxicity on the cytoskeleton of neurotubules and intermediate filaments, Zn wires were implanted into the brains of adult Lewis rats for periods of 1-35 weeks. After 16 weeks of implant, some neurons showed bundles of intermediate filaments which were often localized in the perinuclear area. At the same time, occasional 200 nm tubular-like structures were seen in swollen dendrites. These structures were morphologically similar to Zn ion-induced aggregates of pure tubulin and structures found in ZnSO4-treated dorsal root ganglion organotypic cultures. The 200 nm structures in dendrites and the intermediate filaments in neurons increased in frequency with time. After 35 weeks of Zn wire implant, few microtubules could be found in the lesion. All the animals showed an astrocyte and glial filament proliferation with axoglial membrane specialization. Other wires studied: Pt, Ni, Co, Mg, demonstrate that Zn wires have a specific effect on cytoskeletal proteins in the CNS of the rat and many of the effects can be explained by an interaction between Zn and tubulin.
为了测试体内锌对神经微管和中间丝细胞骨架的神经毒性，将锌丝植入成年Lewis大鼠脑内1至35周。植入16周后，一些神经元显示出中间丝束，这些丝束常位于核周区域。同时，在肿胀的树突中偶尔可见200纳米的管状结构。这些结构在形态上类似于锌离子诱导的纯微管蛋白聚集体以及在硫酸锌处理的背根神经节器官型培养物中发现的结构。树突中的200纳米结构和神经元中的中间丝频率随时间增加。锌丝植入35周后，在损伤部位几乎找不到微管。研究的其他金属丝：铂、镍、钴、镁，表明锌丝对大鼠中枢神经系统的细胞骨架蛋白有特定作用，许多作用可以用锌与微管蛋白之间的相互作用来解释。
