Saleem M, Atkinson B G
Can J Biochem. 1980 Jun;58(6):461-8. doi: 10.1139/o80-061.
Recent in vivo and in vitro studies with polyribosomes from the tail muscle of T3-treated tadpoles establish that this hormone initiates a regulating effect on tadpole tail muscle which operates at the translational level and results in an overall decreased rate of protein synthesis (Saleem, M. & Atkinson, B. G. (1978) J. Biol. Chem. 253, 1378-1384). This hormone-induced decrease in the rate of protein synthesis is partially, if not wholly, due to the presence of a sarcoplasmic factor(s) inhibiting ribosomal translational efficiency. This research employs the use of a reconstituted, cell-free polypeptide synthesizing system as a means to substantiate the presence of an inhibitor and further elucidate the mechanism by which this inhibitory factor(s) depresses protein synthesis. The results of this study further demonstrate the presence of an inhibitor of protein synthesis in the tail muscle sarcoplasm of T3-treated tadpoles and suggest that this depressed synthetic activity results from an interaction of the inhibitor with ribosomal or polyribosomal constituents.
近期对经T3处理的蝌蚪尾部肌肉多核糖体进行的体内和体外研究表明，这种激素对蝌蚪尾部肌肉产生一种调节作用，该作用在翻译水平发挥作用，导致蛋白质合成的总体速率下降（萨利姆，M.和阿特金森，B.G.（1978年）《生物化学杂志》253卷，1378 - 1384页）。这种激素诱导的蛋白质合成速率下降，如果不是完全的话，部分是由于肌浆中存在一种抑制核糖体翻译效率的因子。本研究采用重组的无细胞多肽合成系统来证实抑制剂的存在，并进一步阐明这种抑制因子降低蛋白质合成的机制。这项研究的结果进一步证明了经T3处理的蝌蚪尾部肌肉肌浆中存在蛋白质合成抑制剂，并表明这种合成活性降低是由于抑制剂与核糖体或多核糖体成分相互作用所致。
