表皮生长因子与小鼠表皮细胞原代培养物和永久培养物的结合：促癌佛波酯的抑制作用
Binding of epidermal growth factor to primary and permanent cultures of mouse epidermal cells: inhibition by tumor-promoting phorbol esters.
作者信息
Murray A W, Fusenig N E
出版信息
Cancer Lett. 1979 Jul;7(2-3):71-7. doi: 10.1016/s0304-3835(79)80098-1.
PMID:314329
Abstract
High affinity binding of epidermal growth factor (EGF) was detected with mouse epidermal cells in primary culture and with 2 epidermal cell lines maintained in permanent culture. EGF binding was inhibited by low concentrations of the tumor promoters 12-O-tetradecanoyl phorbol-13-acetate (TPA) and phorbol didecanoate (PDD), but not by the non-promoting phorbol esters 4-O-methyl TPA and 4 alpha-PDD.
摘要
在原代培养的小鼠表皮细胞以及两种永生化培养的表皮细胞系中均检测到表皮生长因子（EGF）的高亲和力结合。低浓度的肿瘤启动子12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）和佛波醇二癸酸酯（PDD）可抑制EGF结合，但非启动性佛波醇酯4 - O - 甲基TPA和4α - PDD则无此作用。
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