Yamamura Y, Tanaka J L, Madyastha K R, Fudenberg H H, Proctor J W
Immunol Commun. 1981;10(1):9-20. doi: 10.3109/08820138109050682.
Two distinct mitogenic subcomponents of phytohemagglutinin (PHA)--leucoagglutinin (LA) and "purified" PHA--apparently stimulated different subpopulations of murine T cells. In the DBA/2J strain, the mitogenic responses of splenic lymphocytes to LA reached maximal levels after 24 to 36 hr exposure and almost completely disappeared by 48 hr, whereas maximal responses to PHA were maintained after 48 hr incubation. The levels of LA-responding T cells were highest in DBA/2J spleens at 5 weeks of age but markedly declined by 9 weeks of age, whereas thymic levels of LA-responding T cells reached a maximum at 9 weeks of age and remained maximal past 15 weeks of age. PHA-responding cells, in contrast, reached maximal levels in both the spleen and thymus of DBA/2J mice at 9 weeks of age. In C57BL/6J mice, splenic and thymic lymphocytes responded similarly to both components, except that the response of splenic lymphocytes to PHA reached a maximum after shorter incubation time and declined sooner than their response to LA. The mitogenic responses of C57BL/6J thymocytes to both components were already at their peak by 5 weeks of age and almost totally disappeared by 9 weeks, whereas the responses of splenic lymphocytes were maximal at 9-15 weeks of age. The responses of DBA/2J splenocytes to LA was significantly augmented by PHA, but LA markedly suppressed the proliferative responses to PHA.
植物血凝素（PHA）的两种不同的促有丝分裂亚组分——白细胞凝集素（LA）和“纯化的”PHA——显然刺激了小鼠T细胞的不同亚群。在DBA/2J品系中，脾淋巴细胞对LA的促有丝分裂反应在暴露24至36小时后达到最高水平，到48小时时几乎完全消失，而对PHA的最大反应在孵育48小时后仍能维持。对LA有反应的T细胞水平在5周龄的DBA/2J脾脏中最高，但在9周龄时显著下降，而胸腺中对LA有反应的T细胞水平在9周龄时达到最大值，并在15周龄后一直保持在最高水平。相比之下，对PHA有反应的细胞在9周龄时在DBA/2J小鼠的脾脏和胸腺中均达到最高水平。在C57BL/6J小鼠中，脾淋巴细胞和胸腺淋巴细胞对这两种组分的反应相似，只是脾淋巴细胞对PHA的反应在较短的孵育时间后达到最大值，且比它们对LA的反应下降得更快。C57BL/6J胸腺细胞对这两种组分的促有丝分裂反应在5周龄时已达到峰值，到9周龄时几乎完全消失，而脾淋巴细胞的反应在9至15周龄时最大。DBA/2J脾细胞对LA的反应被PHA显著增强，但LA明显抑制了对PHA的增殖反应。
