大鼠淋巴结细胞体外抗原诱导增殖的最佳培养条件。
Optimal culture conditions for in vitro antigen-induced proliferation of rat lymph node cells.
作者信息
Holt P G, Leivers S, Warner L A
出版信息
J Immunol Methods. 1981;44(2):205-9. doi: 10.1016/0022-1759(81)90348-3.
Popliteal and inguinal lymph node cells (LNC) from rats immunized with ovalbumin (OA) in Freund's adjuvant, were cultured with OA for 4 days in a variety of serum-supplemented media. Incorporation of [3H]thymidine into [3H]DNA was assessed as an index of antigen-induced lymphoproliferation. Optimal conditions for proliferation were found to comprise culture in flat-bottomed vessels, in the presence of RPMI 1640 medium adjusted to rat osmolality (310 mosmoles/kg) containing 5% v/v fresh syngeneic rat serum plus 4 X 10(-4) M 2-mercaptoethanol.
用弗氏佐剂中的卵清蛋白（OA）免疫的大鼠的腘窝和腹股沟淋巴结细胞（LNC），在多种补充血清的培养基中与OA一起培养4天。将[3H]胸苷掺入[3H]DNA作为抗原诱导的淋巴细胞增殖的指标进行评估。发现增殖的最佳条件包括在平底容器中培养，培养基为调整至大鼠渗透压（310毫渗摩尔/千克）的RPMI 1640培养基，其中含有5%（v/v）新鲜的同基因大鼠血清以及4×10⁻⁴ M 2-巯基乙醇。
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