Altman A, Rapp H J
Int J Cancer. 1978 Nov 15;22(5):595-601. doi: 10.1002/ijc.2910220514.
Guinea-pig lymph-node cells (LNC) cultured for 5 days in medium containing fetal calf serum (FCS) were cytotoxic to various target cells. LNC cultured in medium supplemented with fresh, autologous guinea-pig serum (GPS) instead of FCS were not detectably cytotoxic unless agents that stimulate lymphocyte proliferation were added to the culture medium. The stimulating agents we studied were 2-mercaptoethanol (2-ME), syngeneic tumor cells, allogeneic peritoneal exudate cells (PEC) and the T-cell mitogens concanavalin A (Con A) and phytohemagglutinin (PHA). LNC cultured in the presence of these agents were cytotoxic to normal syngeneic fibroblasts and to syngeneic, allogeneic or xenogeneic tumor cells but not to PHA-induced lymphoblasts. Potentiation of cytotoxicity in vitro was accompanied by a marked proliferation of the cultured LNC; the combination of several stimulatory agents had an additive effect on the generation of cytotoxicity in culture.
在含有胎牛血清（FCS）的培养基中培养5天的豚鼠淋巴结细胞（LNC）对各种靶细胞具有细胞毒性。在添加新鲜自体豚鼠血清（GPS）而非FCS的培养基中培养的LNC，除非向培养基中添加刺激淋巴细胞增殖的试剂，否则未检测到细胞毒性。我们研究的刺激剂有2-巯基乙醇（2-ME）、同基因肿瘤细胞、同种异体腹腔渗出细胞（PEC）以及T细胞有丝分裂原刀豆球蛋白A（Con A）和植物血凝素（PHA）。在这些试剂存在下培养的LNC对正常同基因成纤维细胞以及同基因、同种异体或异种肿瘤细胞具有细胞毒性，但对PHA诱导的淋巴母细胞无细胞毒性。体外细胞毒性的增强伴随着培养的LNC的显著增殖；几种刺激剂的组合对培养中细胞毒性的产生具有相加作用。
