Tamura S I, Kojima A, Egashira Y
Microbiol Immunol. 1981;25(7):693-704. doi: 10.1111/j.1348-0421.1981.tb00072.x.
In vitro exposure to human serum albumin (HSA) of splenic lymphocytes from mice sensitized for delayed-type hypersensitivity (DTH) against HSA resulted in the release of substances that could induce a footpad inflammatory reaction with a maximum of 6 hr after injection into normal mice. The substances were fractionated mainly in a molecular weight range of 30,000 to 70,000 daltons on Sephadex G-200. The ability of sensitized lymphocytes to produce the substances was dependent on T cells, was antigen specific, and correlated well with the ability of the lymphocytes to mediate DTH reactions. Moreover, the substances were produced efficiently by the DTH effector cell population generated in the in vitro culture system and also by the effector cell-enriched fractions on discontinuous bovine serum albumin gradients. These results suggest that the substances are produced by DTH-effector cells.
对已对人血清白蛋白（HSA）致敏以产生迟发型超敏反应（DTH）的小鼠脾淋巴细胞进行体外人血清白蛋白（HSA）暴露，导致释放出一些物质，这些物质在注入正常小鼠后最多6小时可引发足垫炎症反应。这些物质在Sephadex G - 200上主要在分子量30,000至70,000道尔顿范围内分级分离。致敏淋巴细胞产生这些物质的能力取决于T细胞，具有抗原特异性，并且与淋巴细胞介导DTH反应的能力密切相关。此外，在体外培养系统中产生的DTH效应细胞群体以及在不连续牛血清白蛋白梯度上的效应细胞富集级分也能高效产生这些物质。这些结果表明这些物质是由DTH效应细胞产生的。
