Tamura S, Tsuru S, Chiba J, Kojima A
Microbiol Immunol. 1982;26(11):1065-77. doi: 10.1111/j.1348-0421.1982.tb00255.x.
In vitro cultivation of normal mouse spleen cells with human serum albumin generated effector cells that mediate the delayed-type hypersensitivity (DTH) reaction. The cultured cells, when incubated in a serum-free medium for a further 24 hr, released substances (FPRF) which caused a footpad inflammatory reaction at a maximum of 6 hr after injection into normal syngeneic or allogeneic strains of mice, as well as macrophage migration inhibition factor (MIF) and macrophage activating factor (MAF). The DTH-effector cells in the culture were fractionated in the low density layers by discontinuous bovine serum albumin density gradient centrifugation. The effector cells in the low density layers were further enriched in the Lyt 1 subpopulation of T cells when fractionated on a fluorescence activated cell sorter. Cells capable of producing the inflammatory substances (FPRF), MIF and MAF were also enriched in the same fraction containing DTH-effector cells. These results suggest that low density, Lyt 1-positive T cells mediating the DTH reaction produce FPRF as well as MIF and MAF.
用人血清白蛋白对正常小鼠脾细胞进行体外培养可产生介导迟发型超敏反应（DTH）的效应细胞。将培养的细胞在无血清培养基中再孵育24小时后，会释放出一些物质（FPRF），这些物质在注射到同基因或异基因正常小鼠品系的足垫后，最多6小时会引起足垫炎症反应，同时还会释放巨噬细胞移动抑制因子（MIF）和巨噬细胞激活因子（MAF）。通过不连续牛血清白蛋白密度梯度离心，将培养物中的DTH效应细胞分馏到低密度层。当在荧光激活细胞分选仪上进行分馏时，低密度层中的效应细胞在T细胞的Lyt 1亚群中进一步富集。能够产生炎症物质（FPRF）、MIF和MAF的细胞也在含有DTH效应细胞的同一分馏物中富集。这些结果表明，介导DTH反应的低密度、Lyt 1阳性T细胞会产生FPRF以及MIF和MAF。
