Arroyo-Begovich A, Martinez Palomo A, Eugenia Sanchez Pares M
Arch Invest Med (Mex). 1978;9 Suppl 1:105-12.
The presence of chitin in the walls of E. invadens cysts has provided a new way to study the process of encystation, since chitin stains intensely in the presence of Calcofluor white M2R, a fluroescent dye. With this technique we have observed various stages in the differentiation of trophozoite membrane, then the cyst is formed inside the trophozoite and finally the cyst leaves the trophozoite after partial degradation of trophozoite body.
侵袭内变形虫包囊壁中几丁质的存在为研究包囊形成过程提供了一种新方法,因为几丁质在荧光染料荧光增白剂M2R存在的情况下会强烈染色。通过这项技术,我们观察到滋养体膜分化的各个阶段,然后在滋养体内部形成包囊,最后在滋养体部分降解后包囊离开滋养体。