Ching L M, Muraoka S, Miller R G
J Immunol. 1981 Nov;127(5):2156-63.
We describe a system for growing murine colonies containing subsets of functional T cells derived from immature T cell precursors. In contrast with previously described systems in which colony formation appears to depend upon activation and clonal expansion of mature T cells, we obtain comparable or increased numbers of colonies after depletion of T cells from spleen or bone marrow, or on culturing spleen or bone marrow from athymic nude mice. The proportion of cells expressing the T cell surface markers Thy-1, Lyt-1, and Lyt-2 is highly variable from colony to colony, consistent with the colonies arising from immature T cells, which differentiate in the colonies to give rise to various subsets of mature T cells.
我们描述了一种用于培养含有源自未成熟T细胞前体的功能性T细胞亚群的小鼠集落的系统。与先前描述的集落形成似乎依赖于成熟T细胞的激活和克隆扩增的系统不同，我们在从脾脏或骨髓中去除T细胞后，或在培养无胸腺裸鼠的脾脏或骨髓后，获得了相当数量或更多数量的集落。表达T细胞表面标志物Thy-1、Lyt-1和Lyt-2的细胞比例在不同集落之间变化很大，这与集落源自未成熟T细胞一致，这些未成熟T细胞在集落中分化产生各种成熟T细胞亚群。
