Ortaldo J R, Sharrow S O, Timonen T, Herberman R B
J Immunol. 1981 Dec;127(6):2401-9.
We examined highly purified populations of NK cells, T cells, monocytes, and PMN with a variety of monoclonal reagents presumed specific for T cells and monocytes. The enriched NK cells showed a pattern of reactivity that was distinct from that of the other 3 populations, sharing antigens with T cells but also sharing antigens with monocytes and/or PMN. A portion of the enriched NK cells expressed OKT8, OKT10, and Lyt-3 antigens that were reported previously as T cell-associated antigens, but no reactivity was seen with other T cell-associated antigens (OKT3, OKT4, OKT6, and OKT11). In addition a portion of the enriched NK cells were positive for OKM1, asialo-GM1, and Ia antigens, which are undetectable on typical T cells but are present on PMN and/or monocytes. By depletion of antigen-positive cells on antibody monolayers, it was found that the activity of the enriched NK population was associated mainly with cells reactive with OKT10, OKM1, and Lyt-3. Although considerable evidence exists to support the T lineage of NK cells, the sharing of some antigens by NK cells and monocytes has suggested a possible relationship of NK cells to cells of the monocyte lineage. A possibility certainly exists that NK cells are not in either T cell or monocyte lineages and may represent a separate lineage of lymphoid cells.
我们使用了多种推测对T细胞和单核细胞具有特异性的单克隆试剂，检测了高度纯化的自然杀伤细胞（NK细胞）、T细胞、单核细胞和多形核白细胞（PMN）群体。富集的NK细胞呈现出一种与其他3种细胞群体不同的反应模式，它们与T细胞共享抗原，但也与单核细胞和/或PMN共享抗原。一部分富集的NK细胞表达了先前报道为T细胞相关抗原的OKT8、OKT10和Lyt-3抗原，但与其他T细胞相关抗原（OKT3、OKT4、OKT6和OKT11）没有反应。此外，一部分富集的NK细胞对OKM1、去唾液酸GM1和Ia抗原呈阳性，这些抗原在典型的T细胞上无法检测到，但在PMN和/或单核细胞上存在。通过在抗体单层上耗尽抗原阳性细胞，发现富集的NK细胞群体的活性主要与对OKT10、OKM1和Lyt-3有反应的细胞相关。尽管有大量证据支持NK细胞的T细胞谱系，但NK细胞和单核细胞共享一些抗原这一现象表明NK细胞与单核细胞谱系的细胞可能存在某种关系。当然存在一种可能性，即NK细胞既不属于T细胞谱系也不属于单核细胞谱系，可能代表一种独立的淋巴细胞谱系。
