用N-乙基马来酰亚胺解离青蛙嗅觉上皮
Dissociation of frog olfactory epithelium with N-ethylmaleimide.
作者信息
Kleene S J, Gesteland R C
出版信息
Brain Res. 1981 Dec 21;229(2):536-40. doi: 10.1016/0006-8993(81)91018-0.
PMID:6975646
Abstract
Treatment of frog olfactory epithelium with 8 mM N-ethylmaleimide for 2 min results in extensive dissociation of the epithelium. The resulting cell suspension contains single olfactory receptor neurons, sustentacular cells, respiratory epithelial cells, and cells of Bowman's glands. The cells in suspension exhibit the same morphologies seen in histological sections of intact epithelium.
摘要
用8 mM N-乙基马来酰亚胺处理青蛙嗅觉上皮2分钟会导致上皮细胞大量解离。所得细胞悬液包含单个嗅觉受体神经元、支持细胞、呼吸上皮细胞和鲍曼腺细胞。悬液中的细胞呈现出与完整上皮组织学切片中所见相同的形态。
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