嗅觉接收的化学过程：免受巯基试剂抑制的刺激特异性保护。
The chemistry of olfactory reception: stimulus-specific protection from sulfhydryl reagent inhibition.
作者信息
Getchell M L, Gesteland R C
出版信息
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1494-8. doi: 10.1073/pnas.69.6.1494.
Abstract
The group-specific protein reagent, N-ethylmaleimide, irreversibly blocks the electrical response of the olfactory receptor organ of the frog to odorous stimuli. If the odorous substance, ethyl n-butyrate, in concentrations high enough to saturate the receptor system, is present in the nasal cavity before and during a brief exposure to N-ethylmaleimide, the nose, after a wash and a recovery period, responds in nearly normal fashion to vapors of ethyl n-butyrate. Responses to other odorous substances, except those closely related to ethyl n-butyrate, are abolished. We propose that we can use this protection technique to identify the properties of the various receptor sites in the nose, and possibly to characterize the receptor substances.
摘要
组特异性蛋白质试剂N - 乙基马来酰亚胺可不可逆地阻断青蛙嗅觉感受器器官对气味刺激的电反应。如果在短暂暴露于N - 乙基马来酰亚胺之前及期间，鼻腔中存在浓度足以使受体系统饱和的气味物质丁酸乙酯，在冲洗和恢复期后，鼻子对丁酸乙酯蒸气的反应几乎正常。对其他气味物质的反应（与丁酸乙酯关系不紧密的那些除外）则被消除。我们建议可以利用这种保护技术来识别鼻子中各种受体位点的特性，并有可能对受体物质进行表征。
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