慢性淋巴细胞白血病B淋巴细胞中抗坏血酸含量增加。
Increased ascorbic acid content in chronic lymphocytic leukemia B lymphocytes.
作者信息
Liebes L, Krigel R, Kuo S, Nevrla D, Pelle E, Silber R
出版信息
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6481-4. doi: 10.1073/pnas.78.10.6481.
Human lymphocyte extracts analyzed by high-performance liquid chromatography reveal a major UV-absorbing peak that was shown to be ascorbic acid by spectral, chemical, and enzymatic criteria. Because this peak appeared very prominent in the elution profile of chronic lymphocytic leukemia (CLL) lymphocyte extracts, we measured the ascorbic acid content in lymphocytes from the blood of normal subjects and untreated patients with chronic lymphocytic leukemia. A significantly higher concentration of 111 +/- 15.3 nmol per 10(8) cells (mean +/- SEM) was found in CLL lymphocytes than in normal blood lymphocytes, which contained 42.2 +/- 3.3 nmol per 10(8) cells. Selective enrichment with B and T cells showed that this difference was limited to the chronic lymphocytic leukemia B cell, which had a 5- to 15-fold higher content of ascorbic acid than normal B cells had. In contrast, the ascorbic acid level was similar in normal and CLL T cells. The very high ascorbic acid content provides the chronic lymphocytic leukemia B cell with a reducing substance that could react with oxidants or free radicals.
通过高效液相色谱分析的人淋巴细胞提取物显示出一个主要的紫外线吸收峰，根据光谱、化学和酶学标准，该峰被证明是抗坏血酸。由于这个峰在慢性淋巴细胞白血病（CLL）淋巴细胞提取物的洗脱图谱中显得非常突出，我们测量了正常受试者和未经治疗的慢性淋巴细胞白血病患者血液中淋巴细胞的抗坏血酸含量。发现CLL淋巴细胞中每10⁸个细胞的抗坏血酸浓度为111±15.3 nmol（平均值±标准误），显著高于正常血液淋巴细胞，后者每10⁸个细胞含有42.2±3.3 nmol。对B细胞和T细胞进行选择性富集显示，这种差异仅限于慢性淋巴细胞白血病B细胞，其抗坏血酸含量比正常B细胞高5至15倍。相比之下，正常T细胞和CLL T细胞中的抗坏血酸水平相似。非常高的抗坏血酸含量为慢性淋巴细胞白血病B细胞提供了一种可与氧化剂或自由基反应的还原物质。
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