Kumegawa M, Hiramatsu M, Yajima T, Hatakeyama K, Hosoda S, Namba M
Endocrinology. 1982 Feb;110(2):607-12. doi: 10.1210/endo-110-2-607.
Biochemical and morphological studies were made on the effect of epidermal growth factor (EGF), isolated from male mouse submandibular gland, on collagen formation in clone RLC-18(4) epithelial cells from rat liver. EGF did not affect the number of these cells. EGF in the range of 0.5-500 ng/ml caused a dose-dependent increase in the content of hydroxyproline. It also increased the content of acidic glycosaminoglycans (AGAG), which are thought to be closely related to the formation of collagen fibers, and increased the activity of glutamine glucose-6-phosphate aminotransferase, an enzyme for AGAG synthesis but not that of N-acetyl-beta-glucosaminidase, an enzyme for AGAG degradation. It has no significant effect on the protein content of the cells. Studies on the effect of actinomycin D indicated that EGF may enhance de novo synthesis of hydroxyproline in liver epithelial cells and also that of glutamine glucose-6-phosphate aminotransferase, thus increasing the AGAG content of the cells. Antibody to EGF largely blocked collagen formation in the cells, even in the absence of EGF, indicating that EGF or EGF-like substances in the serum may affect collagen formation. In rat liver fibroblasts, EGF had little effect on collagen formation. These results show that EGF may act as a regulatory factor in collagen formation in liver epithelial cells, but not liver mesenchymal cells, in vitro.
对从雄性小鼠颌下腺分离出的表皮生长因子（EGF）对大鼠肝脏克隆RLC - 18(4)上皮细胞中胶原蛋白形成的影响进行了生化和形态学研究。EGF不影响这些细胞的数量。浓度在0.5 - 500 ng/ml范围内的EGF导致羟脯氨酸含量呈剂量依赖性增加。它还增加了酸性糖胺聚糖（AGAG）的含量，AGAG被认为与胶原纤维的形成密切相关，并且增加了谷氨酰胺葡萄糖 - 6 - 磷酸氨基转移酶（一种参与AGAG合成的酶）的活性，但未增加N - 乙酰 - β - 氨基葡萄糖苷酶（一种参与AGAG降解的酶）的活性。它对细胞的蛋白质含量没有显著影响。对放线菌素D作用的研究表明，EGF可能增强肝脏上皮细胞中羟脯氨酸的从头合成以及谷氨酰胺葡萄糖 - 6 - 磷酸氨基转移酶的合成，从而增加细胞的AGAG含量。EGF抗体在很大程度上阻断了细胞中的胶原蛋白形成，即使在没有EGF的情况下也是如此，这表明血清中的EGF或EGF样物质可能影响胶原蛋白的形成。在大鼠肝脏成纤维细胞中，EGF对胶原蛋白形成几乎没有影响。这些结果表明，在体外，EGF可能作为肝脏上皮细胞而非肝脏间充质细胞中胶原蛋白形成的调节因子。
