Ohmori H, Yamamoto I
Immunopharmacology. 1981 Dec;3(4):333-45. doi: 10.1016/0162-3109(81)90026-6.
Seven thiol compounds, namely, 2-mercaptoethanol (2-ME), alpha -thioglycerol (alpha -TG), dithiothreitol (DTT), cysteamine, L-cysteine (Cys), glutathione (GSH), and sodium thioglycollate (TGL) were examined for their mitogenic activities, the effects on mitogen-induced 3H-thymidine uptake, and the effects on antibody synthesis in vitro in murine spleen lymphocytes. All these thiols showed mitogenic activities in RPMI-1640 medium containing 10% fetal calf serum (FCS) with the following order of effectiveness: 2-ME greater than or equal to alpha -TG greater than DTT greater than cysteamine greater than Cys greater than or equal to GSH greater than TGL. A close correlation was observed between the enhancement of the response to lipopolysaccharide (LPS) and the mitogenic activities of these thiol compounds in their magnitude and dose-response profiles. The primary antibody response in vitro to sheep red blood cells (SRBC) (thymus-dependent) or dinitrophenyl (DNP)-Ficoll (thymus-independent) was augmented in a similar fashion by these compounds. T-cells depletion did not influence the enhancement by 2-ME of the antibody response to DNP-Ficoll. There was a discrepancy between the dose-response profiles of mitogenic activities of these compounds and their augmenting effects on antibody responses. Particularly, 2-ME and DTT significantly enhanced the antibody response to DNP-Ficoll or SRBC even at the nonmitogenic doses. Ethyl mercaptan (HSCH2 CH3) showed similar activities to 2-ME, but methylthioethanol (CH3 SCH2 CH2 OH) and ethanol (CH3 CH2 OH) were inactive, thus indicating that the thiol group would play an essential role in the above activities of 2-ME.
研究了七种硫醇化合物，即2-巯基乙醇（2-ME）、α-硫代甘油（α-TG）、二硫苏糖醇（DTT）、半胱胺、L-半胱氨酸（Cys）、谷胱甘肽（GSH）和巯基乙酸钠（TGL）对小鼠脾淋巴细胞的促有丝分裂活性、对有丝分裂原诱导的3H-胸腺嘧啶核苷摄取的影响以及对体外抗体合成的影响。所有这些硫醇在含有10%胎牛血清（FCS）的RPMI-1640培养基中均表现出促有丝分裂活性，其有效性顺序如下：2-ME≥α-TG＞DTT＞半胱胺＞Cys≥GSH＞TGL。在对脂多糖（LPS）反应的增强与这些硫醇化合物的促有丝分裂活性的大小和剂量反应曲线之间观察到密切相关性。这些化合物以类似方式增强了体外对绵羊红细胞（SRBC）（胸腺依赖性）或二硝基苯基（DNP）-菲可（胸腺非依赖性）的初次抗体反应。T细胞耗竭不影响2-ME对DNP-菲可抗体反应的增强作用。这些化合物的促有丝分裂活性的剂量反应曲线与其对抗体反应的增强作用之间存在差异。特别是，即使在非促有丝分裂剂量下，2-ME和DTT也显著增强了对DNP-菲可或SRBC的抗体反应。乙硫醇（HSCH2 CH3）表现出与2-ME相似的活性，但甲硫乙醇（CH3 SCH2 CH2 OH）和乙醇（CH3 CH2 OH）无活性，因此表明巯基在2-ME的上述活性中起重要作用。
