Surovas V, Pieskus J, Tamosiunas V, Sadauskas P
Thymus. 1982 Jan;4(1):31-43.
T-cell subsets in the peripheral blood and lymphoid organs of normal and chronic lymphocytic leukemia (CLL) cattle were studied. Purified T lymphocytes were examined for the numbers of cells with the receptors for IgM (T mu) and IgG (T gamma). The expression of surface receptors on T mu- and T gamma-cell subsets was determined according to their capacity to bind different numbers of erythrocyte antibody complexes EAM or EAG. During the various times of preincubation of T cells at 37 degrees C, an increased release of receptors on the surface of T mu and partly T gamma cells was observed. For the optimal detection of bovine T mu cells, preincubation at 4 degrees C fro 24 h was necessary. The number of T gamma cells in the peripheral blood (P less than 0.001) and spleen (P less than 0.001) of CLL cattle was higher in comparison with controls. A considerably reduced number of T mu cells in the peripheral blood (P less than 0.001) and lymph nodes (P less than 0.02) of CLL cattle was observed. With the exception of thymus, T gamma cells from the lymphoid organs of CLL cattle had a higher expression of receptors than normal T gamma cells.
对正常牛和慢性淋巴细胞白血病（CLL）牛的外周血及淋巴器官中的T细胞亚群进行了研究。对纯化的T淋巴细胞检测带有IgM受体（Tμ）和IgG受体（Tγ）的细胞数量。根据Tμ和Tγ细胞亚群结合不同数量红细胞抗体复合物EAM或EAG的能力，来测定其表面受体的表达。在37℃对T细胞进行不同时间的预孵育过程中，观察到Tμ细胞表面以及部分Tγ细胞表面的受体释放增加。为了最佳检测牛Tμ细胞，有必要在4℃预孵育24小时。与对照组相比，CLL牛外周血（P<0.001）和脾脏（P<0.001）中的Tγ细胞数量更高。观察到CLL牛外周血（P<0.001）和淋巴结（P<0.02）中的Tμ细胞数量显著减少。除胸腺外，CLL牛淋巴器官中的Tγ细胞比正常Tγ细胞具有更高的受体表达。
