T 集落形成是 Tμ（辅助）淋巴细胞的一种特性的证据。
Evidence that T colony formation is a property of T mu (helper) lymphocytes.
作者信息
Foa R, Lauria F, Catovsky D
出版信息
Clin Exp Immunol. 1980 Oct;42(1):152-5.
The T-colony-forming capacity of different T lymphocyte subsets was studied in normal peripheral blood. Unfractionated lymphocytes (after 'Lymphoprep' separation) gave rise to a mean of 150 +/- 27 . 7 s.d. T colonies per 1 x 10(5) cells, while purified T lymphocytes by sheep RBC rosetting formed 110 +/- 32 . 2 colonies. Two subpopulations of T lymphocytes were further isolated according to the presence of Fc receptors for IgG (T gamma) or IgM (T mu) by ox RBC rosetting. T gamma cells were found to have a very low or absent T colony-forming capacity (23 +/- 26 . 2), while T mu cells produced normal colony numbers (106 +/- 28 . 4). Co-culture experiments showed that T gamma cells do not inhibit the T colony growth of normal T cells in our system. Our findings indicate that in human peripheral blood not all T lymphocytes are capable of forming T colonies and that this property is confined to the T mu (helper) lymphocyte subset.
在正常外周血中研究了不同T淋巴细胞亚群形成T集落的能力。未分离的淋巴细胞（经“淋巴细胞分离液”分离后）每1×10⁵个细胞平均产生150±27.7（标准差）个T集落，而通过绵羊红细胞花环法纯化的T淋巴细胞形成110±32.2个集落。根据对IgG（Tγ）或IgM（Tμ）的Fc受体的存在情况，通过氧化红细胞花环法进一步分离出两个T淋巴细胞亚群。发现Tγ细胞的T集落形成能力非常低或没有（23±26.2），而Tμ细胞产生的集落数正常（106±28.4）。共培养实验表明，在我们的系统中Tγ细胞不抑制正常T细胞的T集落生长。我们的研究结果表明，在人外周血中并非所有T淋巴细胞都能够形成T集落，并且这种特性仅限于Tμ（辅助）淋巴细胞亚群。
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