正常人成纤维细胞分泌β-N-乙酰氨基葡萄糖苷酶同工酶。
Secretion of beta-N-acetylglucosaminidase isoenzymes by normal human fibroblasts.
作者信息
Willcox P
出版信息
Biochem J. 1978 Aug 1;173(2):433-9. doi: 10.1042/bj1730433.
Abstract
- Secretion of the lysosomal enzyme beta-N-acetylglucosaminidase (EC 3.2.1.30) by normal human fibroblast cultures was linear with respect to time up to 96h. 2. Two forms of the A isoenzyme of beta-N-acetylglucosaminidase were found in the culture medium. One form was similar to the isoenzyme found in other extracellular fluids, such as plasma and tears, the other resembled the intracellular (lysosomal) enzyme. The presence of the two isoenzymes in the culture medium appears to reflect two distinct secretory processes. 3. It is suggested that plasma acid hydrolases may be destined for incorporation into lysosomes in a manner analogous to that described for the packaging of lysosomal enzymes by fibroblasts.
摘要
- 正常人成纤维细胞培养物中溶酶体酶β-N-乙酰氨基葡萄糖苷酶（EC 3.2.1.30）的分泌在长达96小时内与时间呈线性关系。2. 在培养基中发现了β-N-乙酰氨基葡萄糖苷酶A同工酶的两种形式。一种形式类似于在其他细胞外液（如血浆和眼泪）中发现的同工酶，另一种类似于细胞内（溶酶体）酶。培养基中两种同工酶的存在似乎反映了两种不同的分泌过程。3. 有人提出，血浆酸性水解酶可能以类似于成纤维细胞包装溶酶体酶的方式注定要并入溶酶体。
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