Sahu S, Lynn W S
Biochem J. 1978 Aug 1;173(2):565-8. doi: 10.1042/bj1730565.
Hyaluronic acid was the only glycosaminoglycan found in the pulmonary secretions of patients with asthma. The compound had a hexuronate/hexosamine molar ratio of about 1:1. Glucosamine constituted over 98% of the hexosamines, the remaining being galactosamine. The compound moved as a single spot with the mobility of standard hyaluronic acid on cellulose acetate electrophoresis, and this spot disappeared after digestion with testicular hyaluronidase. Even after extensive proteolysis and purification, the compound was associated with small amounts of protein, the major amino acids of which were aspartic acid, threonine, serine, glutamic acid, glycine and valine.
透明质酸是在哮喘患者肺分泌物中发现的唯一糖胺聚糖。该化合物的己糖醛酸/己糖胺摩尔比约为1:1。葡糖胺占己糖胺的98%以上,其余为半乳糖胺。该化合物在醋酸纤维素电泳上作为单一斑点移动,其迁移率与标准透明质酸相同,并且该斑点在用睾丸透明质酸酶消化后消失。即使经过广泛的蛋白水解和纯化,该化合物仍与少量蛋白质相关,其中主要氨基酸为天冬氨酸、苏氨酸、丝氨酸、谷氨酸、甘氨酸和缬氨酸。