Szelényi J G, Bartha E, Hollán S R
Br J Haematol. 1982 Feb;50(2):241-5. doi: 10.1111/j.1365-2141.1982.tb01914.x.
Acetylcholinesterase (AchE) activity of normal human peripheral blood lymphocytes was determined by an adaptation of the colorimetric and radiometric techniques described for other cells. The enzyme activity seemed to be correlated to the number of T-lymphocytes in the blood. To decide whether only the T-cells possess AchE activity the lymphocytes were separated on Leucopac filter and on Percoll density gradient. B-lymphocytes had no detectable enzyme activity, while the T-lymphocyte fraction represented the total activity measured in the unseparated sample. The majority of AchE activity could be demonstrated in T-lymphocytes of lower density (TLD). The role of AchE in the plasma membrane of various blood cells is not known. Nevertheless, the enzyme is a good marker of the integrity and functional state of the membrane. The difference observed in AchE activity of the lymphocyte populations seems to be suitable for using it to characterize T-lymphocytes.
通过对描述用于其他细胞的比色法和放射性测定技术进行改进，测定了正常人外周血淋巴细胞的乙酰胆碱酯酶（AchE）活性。该酶活性似乎与血液中T淋巴细胞的数量相关。为了确定是否只有T细胞具有AchE活性，淋巴细胞在白细胞过滤柱和Percoll密度梯度上进行了分离。B淋巴细胞没有可检测到的酶活性，而T淋巴细胞部分代表了未分离样品中测得的总活性。大部分AchE活性可在低密度T淋巴细胞（TLD）中显示。AchE在各种血细胞质膜中的作用尚不清楚。然而，该酶是膜完整性和功能状态的良好标志物。淋巴细胞群体中观察到的AchE活性差异似乎适合用于表征T淋巴细胞。
