细菌光合反应中心中铁位点的X射线吸收研究。
An x-ray absorption study of the iron site in bacterial photosynthetic reaction centers.
作者信息
Bunker G, Stern E A, Blankenship R E, Parson W W
出版信息
Biophys J. 1982 Feb;37(2):539-51. doi: 10.1016/S0006-3495(82)84699-7.
Abstract
Measurements were made of the extended x-ray absorption fine structure (EXAFS) of the iron site in photosynthetic reaction centers from the bacterium Rhodopseudomonas sphaeroides. Forms with two quinones, two quinones with added o-phenanthroline, and one quinone were studied. Only the two forms containing two quinones maintained their integrity and were analyzed. The spectra show directly that the added o-phenanthroline does not chelate the iron atom. Further analysis indicates that the iron is octahedrally coordinated by nitrogen and/or oxygen atoms located at various distances, with the average value of about 2.14 A. The analysis suggests that most of the ligands are nitrogens and that three of the nitrogen ligands belong to histidine rings. This interpretation accounts for several unusual features of the EXAFS spectrum. We speculate that the quinones are bound to the histidine rings in some manner. Qualitative features of the absorption edge spectra also are discussed and are related to the Fe-ligand distance.
摘要
对球形红假单胞菌光合反应中心中铁位点的扩展X射线吸收精细结构（EXAFS）进行了测量。研究了含有两个醌的形式、添加邻菲罗啉的两个醌的形式以及一个醌的形式。只有含有两个醌的两种形式保持了其完整性并进行了分析。光谱直接表明，添加的邻菲罗啉不会螯合铁原子。进一步分析表明，铁由位于不同距离的氮和/或氧原子八面体配位，平均值约为2.14埃。分析表明，大多数配体是氮，其中三个氮配体属于组氨酸环。这种解释说明了EXAFS光谱的几个不寻常特征。我们推测醌以某种方式与组氨酸环结合。还讨论了吸收边光谱的定性特征，并将其与铁-配体距离相关联。
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