Wilson P W, Lawson D E
Biochem J. 1978 Aug 1;173(2):627-31. doi: 10.1042/bj1730627.
1,25-Dihydroxycholecalciferol very rapidly stimulates the incorporation of [4,5-3H]leucine into at least two proteins of the chick intestinal mucosal cells. The smaller of the two proteins has mol.wt. approx. 42000, very similar to actin. Other properties of this protein were investigated, including its solubility in salt solution and its behaviour on gel electrophoresis and isoelectric focusing, and in all cases the 1,25-dihydroxycholecalciferol-stimulated protein was indistinguishable from intestinal actin. It was also shown that the brush borders of mucosal cells contain both beta- and gamma-actin in approximately equal amounts and that both forms of the protein appear to be affected by the hormone. It is concluded therefore that one of the earliest actions of 1,25-dihydroxycholecalciferol is to stimulate the incorporation of leucine into beta- and gamma-actin of the mucosal cells or into two proteins very like them.
1,25 - 二羟基胆钙化醇能非常迅速地刺激[4,5 - ³H]亮氨酸掺入雏鸡小肠黏膜细胞的至少两种蛋白质中。这两种蛋白质中较小的一种分子量约为42000，与肌动蛋白非常相似。对该蛋白质的其他特性进行了研究，包括其在盐溶液中的溶解度、在凝胶电泳和等电聚焦中的行为，在所有情况下，1,25 - 二羟基胆钙化醇刺激产生的蛋白质与肠肌动蛋白无法区分。研究还表明，黏膜细胞的刷状缘含有大致等量的β - 肌动蛋白和γ - 肌动蛋白，并且这两种形式的蛋白质似乎都受到该激素的影响。因此得出结论，1,25 - 二羟基胆钙化醇最早的作用之一是刺激亮氨酸掺入黏膜细胞的β - 肌动蛋白和γ - 肌动蛋白或掺入与其非常相似的两种蛋白质中。
