Delia D, Greaves M F, Newman R A, Sutherland D R, Minowada J, Kung P, Goldstein G
Int J Cancer. 1982 Jan 15;29(1):23-31. doi: 10.1002/ijc.2910290106.
A panel of monoclonal antibodies and other markers (e.g., terminal deoxynucleotidyl transferase, sheep erythrocyte rosettes, peanut agglutinin) have been used in conjunction with flow cytometry and biochemical analysis to monitor the induction of maturation in human thymic (T) leukaemic cell lines by phorbol ester (TPA). Seven cell lines underwent multiple phenotypic alterations in response to TPA but were unresponsive to synthetic thymic hormones (TP5, FTS) or to other compounds (e.g. DMSO, retinoic acid) which induce maturation in other types of leukaemia. The changes parallel those observed in normal T-cell differentiation and partly reflect alterations in glycosyl transferase activity, altered synthesis of proteins and regulation of cell surface receptors (for transferrin) associated with rapid growth and metabolism. These studies further illustrate the reversibility of maturation arrest in human leukaemia and provide support for the view that leukaemia may involve regulatory defects in the coupling of proliferation and maturation. Induction of promotion of terminal differentiation in leukaemic equivalents of T-cell precursors may provide a convenient system for the study of biochemical and molecular events involved in T-cell development and diversification.
一组单克隆抗体和其他标志物（如末端脱氧核苷酸转移酶、绵羊红细胞玫瑰花结、花生凝集素）已与流式细胞术和生化分析结合使用，以监测佛波酯（TPA）对人胸腺（T）白血病细胞系成熟诱导作用。七个细胞系对TPA产生了多种表型改变，但对合成胸腺激素（TP5、FTS）或对其他类型白血病有成熟诱导作用的其他化合物（如二甲基亚砜、视黄酸）无反应。这些变化与正常T细胞分化中观察到的变化相似，部分反映了糖基转移酶活性的改变、蛋白质合成的改变以及与快速生长和代谢相关的细胞表面受体（转铁蛋白受体）的调节。这些研究进一步说明了人类白血病中成熟停滞的可逆性，并支持白血病可能涉及增殖与成熟偶联中的调节缺陷这一观点。在T细胞前体的白血病等效物中诱导促进终末分化可能为研究T细胞发育和多样化所涉及的生化和分子事件提供一个便利的系统。
