Vander-Mallie R, Ishizaka T, Ishizaka K
J Immunol. 1982 May;128(5):2306-12.
Fc epsilon R(+) lymphocytes were demonstrated in BALB/c, C57BL/6 and SJL strains of mice by a rosetting technique. The proportion of Fc epsilon R(+) lymphocytes in their spleens was 25 to 33% of the total cells. The majority of the Fc epsilon R(+) cells in the spleen of BALB/c mice were B cells, which also have Fc gamma R. However, after infection of mice with Nippostrongylus brasiliensis, a significant proportion of T cells also bore Fc epsilon R. The average number of Fc epsilon R per Fc epsilon R(+) cell in normal BALB/c spleens was 5100. These receptors were saturated with IGE after incubation of the cells with 5 micrograms/ml mouse IgE. The forward rate constant (k1) of the IgE binding to Fc epsilon R on normal B cells was 1.66 X 10(4) M-1 sec-1, while the dissociation rate of IgE from the Fc epsilon R was 1.7 x 10(-4) sec-1. The equilibrium constant between mouse IgE and Fc epsilon R on normal B cells was approximately 0.95 X 10(8)M-1. However, Fc epsilon R on mouse lymphocytes appeared to be heterogeneous with respect to their affinity for IgE. After infection of mice with Nippostrongylus brasiliensis, the number of Fc epsilon R per receptor-bearing lymphocyte increased several-fold. The FC epsilon R newly expressed after infection appeared to have a lower affinity for IgE than those present originally.
通过玫瑰花结技术在BALB/c、C57BL/6和SJL品系小鼠中证实了FcεR(+)淋巴细胞。它们脾脏中FcεR(+)淋巴细胞的比例占总细胞数的25%至33%。BALB/c小鼠脾脏中的大多数FcεR(+)细胞是B细胞，这些B细胞也具有FcγR。然而，在用巴西日圆线虫感染小鼠后，相当一部分T细胞也带有FcεR。正常BALB/c脾脏中每个FcεR(+)细胞上FcεR的平均数量为5100个。在用5微克/毫升小鼠IgE孵育细胞后，这些受体被IgE饱和。正常B细胞上IgE与FcεR结合的正向速率常数（k1）为1.66×10⁴M⁻¹秒⁻¹，而IgE从FcεR上的解离速率为1.7×10⁻⁴秒⁻¹。正常B细胞上小鼠IgE与FcεR之间的平衡常数约为0.95×10⁸M⁻¹。然而，小鼠淋巴细胞上的FcεR对IgE而言似乎具有异质性。在用巴西日圆线虫感染小鼠后，每个带有受体的淋巴细胞上FcεR的数量增加了几倍。感染后新表达的FcεR对IgE的亲和力似乎比原来存在的FcεR低。
