在含有十二烷基硫酸钠的聚丙烯酰胺凝胶上进行电泳后对大鼠肠膜酶的鉴定。
The identification of rat intestinal membrane enzymes after electrophoresis on polyacrylamide gels containing sodium dodecyl sulphate.
作者信息
Hauri H P, Green J R
出版信息
Biochem J. 1978 Jul 15;174(1):61-66. doi: 10.1042/bj1740061.
Abstract
Brush-border membranes were isolated from the rat small intestine and then treated with sodium dodecyl sulphate under non-reducing conditions at room temperature. Analysis of the solubilized components by polyacrylamide-gel electrophoresis identified three major glycoproteins that co-migrate with glucoamylase-maltase-sucrase, lactase and isomaltase-maltase-sucrase activities. High activities of alkaline phosphatase and trehalase were detectable, but they could not be attributed to distinct co-migrating protein bands. Analysis of mucosa from the distal small intestine by the same methods showed a pattern of bands different from that obtained with the proximal intestine, which appeared to correlate with the relative deficiency of some of the enzymes in the distal region.
摘要
从大鼠小肠中分离出刷状缘膜，然后在室温下非还原条件下用十二烷基硫酸钠处理。通过聚丙烯酰胺凝胶电泳对溶解成分进行分析，鉴定出三种主要糖蛋白，它们与葡糖淀粉酶 - 麦芽糖酶 - 蔗糖酶、乳糖酶以及异麦芽糖酶 - 麦芽糖酶 - 蔗糖酶活性共同迁移。可检测到碱性磷酸酶和海藻糖酶的高活性，但它们不能归因于明显共同迁移的蛋白条带。用相同方法对远端小肠黏膜进行分析，显示出的条带模式与近端小肠不同，这似乎与远端区域某些酶的相对缺乏相关。
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