Maestracci D, Preiser H, Hedges T, Schmitz J, Crane R K
Biochim Biophys Acta. 1975 Mar 13;382(2):147-56. doi: 10.1016/0005-2736(75)90173-x.
The postition of a number of human intestine brush border membrane enzyme activities in polyacrylamide gels after electrophoresis has been determined. These activities are, in order from the origin, maltase/glucoamylase, lactase/phlorizin hydrolase, maltase/sucrase/isomaltase, enteropeptidase, trehalase and gamma-glutamyl-transferase. Leucylnaphthylamide hydrolyzing activity was inactivated by sodium dodecylsulfate and its position was not determined. The positions of the activities have been correlated with the positions of protein bands previously determined. One such band situated between enteropeptidase and alkaline phosphatase has not been identified.
已确定多种人类小肠刷状缘膜酶活性在聚丙烯酰胺凝胶电泳后的位置。这些活性从原点起依次为麦芽糖酶/葡糖淀粉酶、乳糖酶/根皮苷水解酶、麦芽糖酶/蔗糖酶/异麦芽糖酶、肠肽酶、海藻糖酶和γ-谷氨酰转移酶。亮氨酰萘胺水解活性被十二烷基硫酸钠灭活，其位置未确定。这些活性的位置已与先前确定的蛋白条带位置相关联。位于肠肽酶和碱性磷酸酶之间的一条这样的条带尚未被鉴定出来。
