Simmons J E, Bronsky P T, Woodard E J
Biol Neonate. 1982;41(3-4):148-54. doi: 10.1159/000241544.
The uptake of radioactivity in the adrenal glands of 5-day-old male and female rats was examined 2 h following injection of radiolabeled testosterone, R1881, diethylstilbestrol, or estradiol. The adrenals of both sexes had higher levels of radioactivity than blood after testosterone or synthetic androgen (R 1881) injection. The adrenals of neither sex showed any significant accumulation of radioactivity, following 3H-estradiol injection. When, however, cytosol prepared from neonatal adrenal glands was incubated with 2 nM 3H-estradiol, and the incubate subjected to column chromatography on Sephadex G-100, radioactivity was eluted with the void volume; this peak was absent with the addition of 100-fold unlabeled estradiol to the incubation. The possible role of alpha-fetoprotein in preventing the in vivo uptake and retention of 17 beta-estradiol is discussed since 3H-diethylstilbestrol, an estrogen which has low affinity for this protein, was retained by the adrenals of male rats. It is concluded that estrogens or androgens can have a direct effect on the neonatal adrenal gland.
在注射放射性标记的睾酮、R1881、己烯雌酚或雌二醇2小时后，检测了5日龄雄性和雌性大鼠肾上腺对放射性的摄取情况。注射睾酮或合成雄激素（R1881）后，两性大鼠肾上腺的放射性水平均高于血液。注射³H-雌二醇后，两性大鼠的肾上腺均未显示出任何明显的放射性积累。然而，当用2 nM³H-雌二醇孵育新生大鼠肾上腺制备的胞质溶胶，并将孵育物在Sephadex G-100上进行柱色谱分析时，放射性物质与空体积一起被洗脱；在孵育物中加入100倍未标记的雌二醇后，该峰消失。由于³H-己烯雌酚（一种对该蛋白亲和力较低的雌激素）被雄性大鼠的肾上腺保留，因此讨论了甲胎蛋白在防止体内摄取和保留17β-雌二醇方面的可能作用。结论是，雌激素或雄激素可对新生大鼠肾上腺产生直接影响。
