球形红假单胞菌中的短链酰基辅酶A合成酶

Short-chain acyl-coenzyme A synthetases in Rhodopseudomonas sphaeroides.

作者信息

Maruyama K

出版信息

J Biochem. 1982 Feb;91(2):725-30. doi: 10.1093/oxfordjournals.jbchem.a133745.

DOI:10.1093/oxfordjournals.jbchem.a133745

Abstract

Two short-chain fatty acyl-CoA synthetases were extracted from the photosynthetic bacterium, Rhodopseudomonas sphaeroides, and partially purified by column chromatography on Sephacryl S-200 and DEAE-cellulose. One enzyme activated propionate, valerate, acrylate, butyrate, and acetate, and was designated as propionyl-CoA synthetase, since the highest activity and lowest Km value (0.6 mM) were observed with propionate. The other enzyme activated acetate, propionate and acrylate. It showed the highest activity and lowest Km value (0.37 mM) for acetate, and was identified as acetyl-CoA synthetase [EC 6.2.1.1].

摘要

从光合细菌球形红假单胞菌中提取了两种短链脂肪酰辅酶A合成酶，并通过在Sephacryl S - 200和DEAE - 纤维素上进行柱色谱法进行了部分纯化。一种酶可激活丙酸、戊酸、丙烯酸、丁酸和乙酸，由于对丙酸观察到最高活性和最低Km值（0.6 mM），因此被指定为丙酰辅酶A合成酶。另一种酶可激活乙酸、丙酸和丙烯酸。它对乙酸表现出最高活性和最低Km值（0.37 mM），并被鉴定为乙酰辅酶A合成酶[EC 6.2.1.1]。