Medof M E
J Lab Clin Med. 1982 May;99(5):678-92.
A solid phase radioassay for measurement of ICs in biological fluids is described in which ICs present in test sample bind to C1q immobilized on latex particles and bound complexes are quantitated by reaction with radioiodinated mRF. The radioassay can reproducibly measure 10 ng of aggregated human IgG in serum and differentiate soluble complexes from IC-like materials that precipitate with centrifugation or low temperature or stick to test tube walls. Reagents used in the assay, including C1q-L, can be stored for extended periods of time before use. One hundred four of 171 sera from patients with SLE and 8 of 50 sera from patients with LC, assayed by this method, contained elevated levels of ICs relative to controls . IC levels determined by this method correlated with IC data generated by 125I-C1q-PEG precipitation. Raji cell radioimmune assay, and solid-phase conglutinin assay, in some cases but not other.
本文描述了一种用于测量生物体液中免疫复合物（ICs）的固相放射分析方法。在该方法中，测试样品中的ICs与固定在乳胶颗粒上的C1q结合，然后通过与放射性碘化单克隆类风湿因子（mRF）反应来定量结合的复合物。该放射分析方法能够可重复地测量血清中10 ng的聚集人IgG，并能区分可溶性复合物与通过离心、低温沉淀或粘附在试管壁上的类IC物质。该分析方法中使用的试剂，包括C1q-L，在使用前可长期保存。用该方法检测的171例系统性红斑狼疮（SLE）患者血清中有104例，50例狼疮性肾炎（LC）患者血清中有8例，其ICs水平相对于对照组升高。在某些情况下，通过该方法测定的IC水平与125I-C1q-聚乙二醇（PEG）沉淀法、拉吉细胞放射免疫分析和固相凝集素分析所产生的IC数据相关，但在其他情况下并非如此。
