低浓度伴刀豆球蛋白A对小鼠淋巴细胞的刺激作用。I. 腹腔渗出细胞的作用
Concanavalin A stimulation of mouse lymphocytes at low concentration. I. The effect of peritoneal exudate cells.
作者信息
Young B
出版信息
Immunology. 1982 May;46(1):31-6.
The results presented here show that concanavalin A (Con A) stimulated mouse spleen lymphocytes cultured at 1/10th optimal concentration incorporated less [3H]-TdR than those cultured at the optimal concentration. It was possible to increase the response of 1/10th optimal concentration lymphocytes by supplementing the cultures with macrophage-rich peritoneal exudate cell (PEC) preparations. This effect was dependent on the PEC concentration. [3H]-TdR incorporation by lymphocytes at optimal concentration was inhibited by the addition of PEC to the cultures. Thymocyte cultures at 1/10th optimal concentration also showed increased [3H]-TdR incorporation when supplemented with PEC but there was no inhibition effect at optimal concentration. PEC which had been pretreated with fresh mitomycin C to prevent cell division were as effective as controls at promoting the stimulation of 1/10th optimal concentration lymphocytes. PEC which had been killed were much less effective than controls.
此处呈现的结果表明，在1/10最佳浓度下培养的伴刀豆球蛋白A（Con A）刺激的小鼠脾淋巴细胞比在最佳浓度下培养的淋巴细胞掺入的[³H]-胸腺嘧啶核苷（[³H]-TdR）更少。通过用富含巨噬细胞的腹腔渗出细胞（PEC）制剂补充培养物，可以增强1/10最佳浓度淋巴细胞的反应。这种效应取决于PEC的浓度。向培养物中添加PEC会抑制最佳浓度下淋巴细胞的[³H]-TdR掺入。在1/10最佳浓度下培养的胸腺细胞培养物在补充PEC时也显示出[³H]-TdR掺入增加，但在最佳浓度下没有抑制作用。用新鲜丝裂霉素C预处理以防止细胞分裂的PEC在促进1/10最佳浓度淋巴细胞的刺激方面与对照一样有效。已杀死的PEC的效果比对照差得多。
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