Jensen J R, Threstrup-Pedersen K
Acta Pathol Microbiol Immunol Scand C. 1982 Apr;90(2):109-16. doi: 10.1111/j.1699-0463.1982.tb01426.x.
Tuberculin skin testing with purified protein derivative (PPD) was found to induce alterations of in vitro measures of cell mediated immunity. T lymphopenia with proportionate decreases of TM and TG cell numbers were observed two days after skin testing. A relative augmentation of TM cells was demonstrated after one week. Peripheral blood mononuclear cells from negative skin responders had normal in vitro reactivity to PPD stimulation suggesting a cutaneous abberation in these individuals. Macrophage dependent depressed PHA reactivity of mononuclear cells was demonstrated two days after skin testing. No change in natural killer cell cytotoxicity was observed. Cytotoxicity was positively correlated to the number of TG cells in the assay.
发现用纯化蛋白衍生物（PPD）进行结核菌素皮肤试验可诱导细胞介导免疫的体外检测指标发生改变。皮肤试验两天后观察到T淋巴细胞减少，TM和TG细胞数量成比例下降。一周后显示TM细胞相对增加。皮肤反应阴性者的外周血单核细胞对PPD刺激具有正常的体外反应性，表明这些个体存在皮肤异常。皮肤试验两天后证实单核细胞的巨噬细胞依赖性PHA反应性降低。未观察到自然杀伤细胞细胞毒性的变化。细胞毒性与检测中TG细胞的数量呈正相关。
