Rosenson R S, Flaherty L, Levine H, Reinisch C L
J Immunol. 1982 Jul;129(1):382-7.
Qa2+ tumor cell lines were previously isolated from individual BALB/cBy (Qa2-) splenic lymphomas induced by murine sarcoma virus-murine leukemia virus-Moloney (MSV-MuLV-M). Two clonally derived cell lines, ORA I-a and Thorbly, and one noncloned cell line, BOMS, expressed Qa2, but neither Ly-1 nor Ly-2 were detected. In order to determine whether eight cloned and two noncloned tumor cell lines all represented a unique population of transformed cells, the presence of a series of surface differentiation antigens as studied. In addition to Qa2, each cell line examined expressed IAd, IEd, H-2Kd, H-2Dd, and receptors for C3b and the Fc portion of immunoglobulin (Ig). Neither Thy-1.2 nor Ig were detected on the cell surfaces, and cytoplasmic Ig was not precipitated from metabolically radiolabeled and detergent-solubilized cell extracts. However, monocyte specific alpha-napththyl acetate esterase-containing granules were present in all cell lines examined. Therefore, a unique Qa2+ monocytic cell is repeatedly isolated after chronic MSV-MuLV-M infection. Further analysis of these cells may provide insight into both the regulation of Qa2 expression and the interactions between monocytes and lymphocytes during leukemogenesis.
Qa2+肿瘤细胞系先前是从由鼠肉瘤病毒-鼠白血病病毒-莫洛尼（MSV-MuLV-M）诱导的个体BALB/cBy（Qa2-）脾淋巴瘤中分离出来的。两个克隆衍生的细胞系ORA I-a和Thorbly，以及一个未克隆的细胞系BOMS表达Qa2，但未检测到Ly-1和Ly-2。为了确定八个克隆的和两个未克隆的肿瘤细胞系是否都代表了一个独特的转化细胞群体，研究了一系列表面分化抗原的存在情况。除了Qa2，每个检测的细胞系都表达IAd、IEd、H-2Kd、H-2Dd以及C3b和免疫球蛋白（Ig）Fc部分的受体。在细胞表面未检测到Thy-1.2和Ig，并且从代谢放射性标记和去污剂溶解的细胞提取物中未沉淀出细胞质Ig。然而，在所检测的所有细胞系中都存在含有单核细胞特异性α-萘乙酸酯酶的颗粒。因此，在慢性MSV-MuLV-M感染后反复分离出一种独特的Qa2+单核细胞。对这些细胞的进一步分析可能会为Qa2表达的调节以及白血病发生过程中单核细胞与淋巴细胞之间的相互作用提供深入了解。
