Kim K J, Weinbaum F I, Mathieson B J, McKeever P E, Asofsky R
J Immunol. 1978 Jul;121(1):339-44.
Transplanted lymphomas (Thy 1.2+, Ig-) of BALB/c mice, induced by the injection of 1-ethyl-1-nitrosourea, were adapted for growth as in vitro lines to provide potential tools for investigation of T lymphocyte differentiation and functions. All these tissue culture lines maintained the same pattern of surface differentiation antigens (Ly, TL, and Thy-1 antigens) as they had expressed during in vivo passages: BALENTL 13 was Thy 1.2+, TL.2-, and Ly 1+2-. BALENTL 3, 4, 5, 6, 7, 8, and 14 were Thy 1.2+, TL.2+, and Ly 1-2+. P1798 and BALENTL 9 were Thy 1.2+, TL.2+, and Ly 1-2+. There were various levels of terminal transferase activity present among these T cell tumor lines. The range of variation was from 4.6 units/10(8) cells to 29.3 units/10(8) cells (normal thymocytes, 5.0 units/10(8) cells). This 6-fold variation in TdT activity was present even among those cell lines which were Ly 1-2+, TL+. Most cultures lines had chromosome numbers near 40 and generation times of 11 to 22 hr. There were no significant morphologic changes after the adaptation of these tumors in culture except an increase in cytoplasmic C-type virus particles.
通过注射1-乙基-1-亚硝基脲诱导的BALB/c小鼠移植性淋巴瘤（Thy 1.2+，Ig-）被培养成体外细胞系，以提供研究T淋巴细胞分化和功能的潜在工具。所有这些组织培养细胞系保持了与体内传代时相同的表面分化抗原模式（Ly、TL和Thy-1抗原）：BALENTL 13为Thy 1.2+，TL.2-，Ly 1+2-。BALENTL 3、4、5、6、7、8和14为Thy 1.2+，TL.2+，Ly 1-2+。P1798和BALENTL 9为Thy 1.2+，TL.2+，Ly 1-2+。这些T细胞肿瘤细胞系中存在不同水平的末端转移酶活性。变化范围为4.6单位/10⁸细胞至29.3单位/10⁸细胞（正常胸腺细胞为5.0单位/10⁸细胞）。即使在那些Ly 1-2+、TL+的细胞系中，TdT活性也存在6倍的差异。大多数培养细胞系的染色体数接近40，倍增时间为11至22小时。这些肿瘤在培养适应后除了细胞质C型病毒颗粒增加外，没有明显的形态学变化。
