乙酰腺苷酸对去乙酰柠檬酸裂解酶的非酶促再活化。化学营养修饰激活酶的首个实例。
Nonenzymatic reactivation of des-acetyl citrate lyase by acetyl adenylate. First example of enzyme activation by chemotrophic modification.
作者信息
Camici G, Manao G, Ramponi G
出版信息
Physiol Chem Phys. 1975;7(5):409-14.
PMID:698
Abstract
The experimental conditions of nonenzymatic reactivation of des-acetyl citrate lyase from K. aerogenes were studied. It was found that at pH 8.5 0.2 MM acetyl AMP causes a fast reactivation of the enzyme. The pH dependence of activity and the Km for citrate are very similar for both native and reactivated enzyme.
摘要
对产气克雷伯氏菌去乙酰柠檬酸裂合酶的非酶促再活化实验条件进行了研究。结果发现，在pH 8.5、0.2 mM乙酰AMP的条件下，该酶能快速再活化。天然酶和再活化酶的活性pH依赖性以及柠檬酸的米氏常数非常相似。
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