脱乙酰柠檬酸裂解酶的酶促乙酰化机制。
Mechanism of enzymic acetylation of des-acetyl citrate lyase.
作者信息
Schmellenkamp H, Eggerer H
出版信息
Proc Natl Acad Sci U S A. 1974 May;71(5):1987-91. doi: 10.1073/pnas.71.5.1987.
Abstract
A new enzyme, acetate:SH-[acyl-carrier-protein] enzyme ligase (AMP), has been purified about 30-fold from cell-free extracts of Klebsiella aerogenes. The enzyme, in the presence of acetate and ATP, catalyzes the acetylation of enzymically inactive des-acetyl citrate lyase to enzymically active citrate lyase (EC 4.1.3.6). Acetate and ATP could be replaced by acetyl adenylate. Acetyl-CoA can act as acetyl donor in this activation only if trace amounts of adenine nucleotides and auxiliary enzymes are present. These allow formation of acetyl adenylate in acetate- and ATP-generating reactions.
摘要
一种新的酶，即乙酸：SH-[酰基载体蛋白]酶连接酶（AMP），已从产气克雷伯菌的无细胞提取物中纯化出来，纯化倍数约为30倍。该酶在乙酸和ATP存在的情况下，催化无酶活性的去乙酰柠檬酸裂合酶乙酰化，生成有酶活性的柠檬酸裂合酶（EC 4.1.3.6）。乙酸和ATP可用乙酰腺苷酸替代。只有当存在微量腺嘌呤核苷酸和辅助酶时，乙酰辅酶A才能在此激活过程中作为乙酰供体。这些物质能在生成乙酸和ATP的反应中促使乙酰腺苷酸的形成。
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