Collins A, Johnson R
Nucleic Acids Res. 1979 Nov 10;7(5):1311-20. doi: 10.1093/nar/7.5.1311.
In addition to inhibiting replicative DNA synthesis in HeLa cells, novobiocin has a severe effect on the cellular response to UV irradiation, reducing the number of breaks made in pre-existing DNA by the excision repair process. The inhibition of UV repair by novobiocin is reflected in enhanced UV-killing of these cells. Rejoining of DNA after X irradiation is not impaired by novobiocin. The recognition and removal of UV damage may require unwinding of the DNA by gyrase, which--in bacteria--is the target for novobiocin.
除了抑制HeLa细胞中的复制性DNA合成外,新生霉素对细胞对紫外线照射的反应有严重影响,减少了切除修复过程中在已有DNA上产生的断裂数量。新生霉素对紫外线修复的抑制作用表现为这些细胞的紫外线杀伤增强。X射线照射后DNA的重新连接不受新生霉素的影响。紫外线损伤的识别和去除可能需要由促旋酶解开DNA,而在细菌中,促旋酶是新生霉素的作用靶点。