通过与大鼠骨髓前脂肪细胞系REC A16进行体外共培养来诱导大鼠骨髓单核细胞白血病细胞系c-WRT-7分化。
Differentiation of the rat myelomonocytic leukemia cell line c-WRT-7 by in vitro culture with the rat bone marrow preadipocyte cell line REC A16.
作者信息
Tanaka T, Okamura S, Yasumoto S, Takeichi N, Kobayashi H, Niho Y
机构信息
Cancer Center, Kyushu University Hospital, Japan.
出版信息
J Cancer Res Clin Oncol. 1993;119(6):335-41. doi: 10.1007/BF01208841.
Differentiation of the rat myelomonocytic leukemia cell line (c-WRT-7) was investigated, by co-culture with a rat embryonic bone marrow preadipose cell line (REC A16). Co-cultivation with REC A16, or with conditioned medium from REC A16 cultures (REC-CM), induced differentiation of c-WRT-7 cells to macrophages. A soluble factor(s) produced by REC A16 appeared to be responsible for the differentiation of c-WRT-7. Because REC-CM was associated with colony-stimulating activity on murine marrow progenitors, c-WRT-7 cells were cultured with various colony-stimulating factors (CSF) and it was found that macrophage CSF (M-CSF) significantly induced differentiation of c-WRT-7. We further demonstrated that both the colony-stimulating and differentiation-inducing activities of REC-CM were significantly blocked by anti-M-CSF antiserum. These results suggest that the differentiation of c-WRT-7 is due to M-CSF produced by REC A16. Co-culture of these two cell lines should provide a useful model to study the mechanisms of interaction between leukemia cells and marrow stroma.
通过与大鼠胚胎骨髓前脂肪细胞系（REC A16）共培养，对大鼠骨髓单核细胞白血病细胞系（c-WRT-7）的分化进行了研究。与REC A16或REC A16培养物的条件培养基（REC-CM）共培养，可诱导c-WRT-7细胞分化为巨噬细胞。REC A16产生的一种或多种可溶性因子似乎是c-WRT-7分化的原因。由于REC-CM与对小鼠骨髓祖细胞的集落刺激活性相关，因此将c-WRT-7细胞与各种集落刺激因子（CSF）一起培养，发现巨噬细胞集落刺激因子（M-CSF）可显著诱导c-WRT-7的分化。我们进一步证明，抗M-CSF抗血清可显著阻断REC-CM的集落刺激和分化诱导活性。这些结果表明，c-WRT-7的分化是由于REC A16产生的M-CSF所致。这两种细胞系的共培养应为研究白血病细胞与骨髓基质之间的相互作用机制提供一个有用的模型。
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