Uede T, Huff T F, Ishizaka K
J Immunol. 1982 Oct;129(4):1384-90.
Rats were immunized with keyhole limpet hemocyanin (KLH) included in complete Freund's adjuvant (CFA) or with the same antigen absorbed to aluminum hydroxide gel. Incubation of their spleen and MLN cells with homologous antigen resulted in the formation of IgE-binding factors. The nature of IgE-binding factors formed by antigenic stimulation was different depending on the adjuvant employed for priming and the period after immunization. If the lymphoid tissues were obtained 2 to 4 wk after antigen priming, IgE-binding factors derived from lymphoid tissues of KLH-alum-primed animals selectively potentiated the IgE response, whereas those produced by the lymphoid tissues of KLH-CFA-primed animals suppressed the IgE response. Analysis of purified IgE-binding factors from KLH-primed spleen cells revealed that the factors were heterogeneous with respect to their m.w., and that IgE-binding factors of a m.w. of 13,000 were responsible for the biologic activities. Thus, the 13,000-dalton factors obtained from KLH-alum-primed spleen cells selectively potentiated the IgE response, and those from KLH-CFA-primed spleen cells suppressed the IgE response. The selective formation of either IgE-potentiating factors or IgE-suppressive factors by KLH-primed spleen cells was observed only when the cells were stimulated by specific antigen. When the same KLH-primed spleen cells were incubated with IgE, IgE-binding factors formed in the cultures neither potentiated nor suppressed the IgE response. It was also found that stimulation of a mixture of BCG-primed spleen cells and KLH-alum-primed spleen cells with PPD resulted in the formation of IgE-suppressive factors; the stimulation of the same mixed cell suspension with KLH resulted in the formation of IgE-potentiating factors. The results collectively indicate that the nature of IgE-binding factors is decided by antigen-primed cells.
用完全弗氏佐剂（CFA）中包含的钥孔戚血蓝蛋白（KLH）或吸附于氢氧化铝凝胶的相同抗原对大鼠进行免疫。用同源抗原孵育它们的脾细胞和肠系膜淋巴结（MLN）细胞会导致IgE结合因子的形成。抗原刺激形成的IgE结合因子的性质因用于初次免疫的佐剂和免疫后的时间不同而有所差异。如果在抗原初次免疫后2至4周获得淋巴组织，来自KLH-明矾免疫动物淋巴组织的IgE结合因子会选择性增强IgE反应，而来自KLH-CFA免疫动物淋巴组织产生的因子则会抑制IgE反应。对来自KLH免疫脾细胞的纯化IgE结合因子的分析表明，这些因子在分子量方面具有异质性，分子量为13,000的IgE结合因子具有生物学活性。因此，从KLH-明矾免疫脾细胞获得的13,000道尔顿因子选择性增强IgE反应，而从KLH-CFA免疫脾细胞获得的因子则抑制IgE反应。仅当细胞受到特异性抗原刺激时，才会观察到KLH免疫的脾细胞选择性形成IgE增强因子或IgE抑制因子。当相同的KLH免疫脾细胞与IgE一起孵育时，培养物中形成的IgE结合因子既不增强也不抑制IgE反应。还发现，用结核菌素纯蛋白衍生物（PPD）刺激卡介苗（BCG）免疫的脾细胞和KLH-明矾免疫的脾细胞的混合物会导致IgE抑制因子的形成；用KLH刺激相同的混合细胞悬液会导致IgE增强因子的形成。这些结果共同表明，IgE结合因子的性质由抗原免疫的细胞决定。
