• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

三嗪染料亲和基质上3-羟基丁酸脱氢酶和苹果酸脱氢酶的快速纯化

The rapid purification of 3-hydroxybutyrate dehydrogenase and malate dehydrogenase on triazine dye affinity matrices.

作者信息

Scawen M D, Darbyshire J, Harvey M J, Atkinson T

出版信息

Biochem J. 1982 Jun 1;203(3):699-705. doi: 10.1042/bj2030699.

DOI:10.1042/bj2030699
PMID:6981412
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1158286/
Abstract

3-Hydroxybutyrate dehydrogenase (EC 1.1.1.30) and malate dehydrogenase (EC 1.1.1.37) were purified to homogeneity on a large scale involving only two sequential affinity-chromatography steps on two triazine dye-Sepharose matrices. Recoveries of both enzymes were in excess of 60%. Malate dehydrogenase could also be purified by a combination of triazine dye affinity chromatography and gel filtration on Ultrogel AcA-44, but this offered no significant advantage over the purely affinity procedure.

摘要

3-羟基丁酸脱氢酶（EC 1.1.1.30）和苹果酸脱氢酶（EC 1.1.1.37）通过仅在两种三嗪染料-琼脂糖凝胶基质上进行两个连续的亲和色谱步骤进行大规模纯化至均一性。两种酶的回收率均超过60%。苹果酸脱氢酶也可以通过三嗪染料亲和色谱和在Ultrogel AcA-44上进行凝胶过滤相结合的方法进行纯化，但与纯亲和方法相比，这并没有显著优势。

相似文献

1
The rapid purification of 3-hydroxybutyrate dehydrogenase and malate dehydrogenase on triazine dye affinity matrices.三嗪染料亲和基质上3-羟基丁酸脱氢酶和苹果酸脱氢酶的快速纯化
Biochem J. 1982 Jun 1;203(3):699-705. doi: 10.1042/bj2030699.
2
Affinity chromatography on immobilized dyes.固定化染料亲和层析
Methods Enzymol. 1984;104:97-113. doi: 10.1016/s0076-6879(84)04085-4.
3
Large-scale purification of enzymes.酶的大规模纯化
Philos Trans R Soc Lond B Biol Sci. 1983 Jan 26;300(1100):249-61. doi: 10.1098/rstb.1983.0003.
4
Biomimetic-dye affinity chromatography for the purification of mitochondrial L-malate dehydrogenase from bovine heart.用于从牛心纯化线粒体L-苹果酸脱氢酶的仿生染料亲和色谱法。
J Biotechnol. 1996 Mar 8;45(3):185-94. doi: 10.1016/0168-1656(95)00156-5.
5
Malate dehydrogenase in phototrophic purple bacteria: purification, molecular weight, and quaternary structure.光合紫色细菌中的苹果酸脱氢酶：纯化、分子量及四级结构
J Bacteriol. 1987 Sep;169(9):4196-202. doi: 10.1128/jb.169.9.4196-4202.1987.
6
Biomimetic dye affinity chromatography for the purification of bovine heart lactate dehydrogenase.用于纯化牛心乳酸脱氢酶的仿生染料亲和色谱法。
J Chromatogr A. 1995 Dec 1;718(1):35-44. doi: 10.1016/0021-9673(95)00661-3.
7
[Isolation and purification of malate dehydrogenase isoforms from phototrophic purple bacteria Rhodobacter sphaeroides and Rhodopseudomonas palustris].[从光合紫色细菌球形红杆菌和沼泽红假单胞菌中分离纯化苹果酸脱氢酶同工型]
Izv Akad Nauk Ser Biol. 2008 Nov-Dec(6):680-7.
8
Separation of malate dehydrogenase isoenzymes by affinity chromatography on 5'-AMP-Sepharose.通过5'-AMP-琼脂糖亲和层析法分离苹果酸脱氢酶同工酶。
Eur J Biochem. 1976 Dec;71(1):25-32. doi: 10.1111/j.1432-1033.1976.tb11085.x.
9
Purification and characterization of a bifunctional L-(+)-tartrate dehydrogenase-D-(+)-malate dehydrogenase (decarboxylating) from Rhodopseudomonas sphaeroides Y.球形红假单胞菌Y中双功能L-(+)-酒石酸脱氢酶-D-(+)-苹果酸脱氢酶（脱羧）的纯化与特性分析
J Bacteriol. 1983 Jul;155(1):281-90. doi: 10.1128/jb.155.1.281-290.1983.
10
A facile method for the isolation of porcine heart mitochondrial malate dehydrogenase by affinity elution chromatography on Procion Red HE3B.一种通过在普施安红HE3B上进行亲和洗脱色谱法分离猪心线粒体苹果酸脱氢酶的简便方法。
Biosci Rep. 1983 Nov;3(11):1035-43. doi: 10.1007/BF01121030.

引用本文的文献

1
Purification and characterization of an (S)-3-hydroxycarboxylate oxidoreductase from Clostridium tyrobutyricum.来自酪丁酸梭菌的(S)-3-羟基羧酸盐氧化还原酶的纯化与特性分析
Appl Microbiol Biotechnol. 1994 Oct;42(1):40-5. doi: 10.1007/BF00170222.
2
Tandem dye-ligand chromatography and biospecific elution applied to the purification of glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides.串联染料配体色谱法和生物特异性洗脱应用于从肠系膜明串珠菌中纯化葡萄糖-6-磷酸脱氢酶。
Biochem J. 1983 Feb 1;209(2):363-71. doi: 10.1042/bj2090363.
3
Malate dehydrogenase in phototrophic purple bacteria: purification, molecular weight, and quaternary structure.光合紫色细菌中的苹果酸脱氢酶：纯化、分子量及四级结构
J Bacteriol. 1987 Sep;169(9):4196-202. doi: 10.1128/jb.169.9.4196-4202.1987.
4
The purification and characterization of glucokinase from the thermophile Bacillus stearothermophilus.嗜热脂肪芽孢杆菌中葡萄糖激酶的纯化与特性分析
Biochem J. 1986 Jul 15;237(2):415-20. doi: 10.1042/bj2370415.
5
Development of monoclonal antibodies to parathyroid hormone-induced resorptive factors from osteoblast-like cells.抗成骨细胞样细胞中甲状旁腺激素诱导的吸收因子单克隆抗体的研制。
Calcif Tissue Int. 1992 Mar;50(3):237-44. doi: 10.1007/BF00296288.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Enzymic determination of D(-)-beta-hydroxybutyric acid and acetoacetic acid in blood.血液中D(-)-β-羟基丁酸和乙酰乙酸的酶法测定
Biochem J. 1962 Jan;82(1):90-6. doi: 10.1042/bj0820090.
3
A cold-sensitive D(-) beta-hydroxybutyric acid dehydrogenase from Rhodospirillum rubrum.来自红螺菌的一种冷敏感型D(-)β-羟基丁酸脱氢酶。
J Biol Chem. 1962 Feb;237:603-7.
4
Purification and properties of malate dehydrogenase from the extreme thermophile Bacillus caldolyticus.嗜热栖热芽孢杆菌苹果酸脱氢酶的纯化及性质
Orig Life. 1980 Jun;10(2):185-92. doi: 10.1007/BF00928668.
5
Triazine-dye affinity; chromatography.三嗪染料亲和作用；色谱法。
Biochem Soc Trans. 1981 Aug;9(4):290-3. doi: 10.1042/bst0090290.
6
Some preparative and analytical applications of triazine dyes.三嗪染料的一些制备和分析应用。
Int J Biochem. 1981;13(1):33-40. doi: 10.1016/0020-711x(81)90133-6.
7
Malate dehydrogenase from thermophilic and mesophilic bacteria. Molecular size, subunit structure, amino acid composition, immunochemical homology, and catalytic activity.
Biochemistry. 1980 May 13;19(10):2017-22. doi: 10.1021/bi00551a002.
8
Malate dehydrogenases. I. A survey of molecular size measured by gel filtration.苹果酸脱氢酶。I. 通过凝胶过滤法测定分子大小的研究。
Biochemistry. 1967 Feb;6(2):603-10. doi: 10.1021/bi00854a031.
9
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
10
Malate dehydrogenases. II. Purification and properties of Bacillus subtilis, Bacillus stearothermophilus, and Escherichia coli malate dehydrogenases.
J Biol Chem. 1967 Apr 10;242(7):1548-59.