新生大鼠长骨中24,25-二羟胆钙化醇受体的证据。
Evidence for 24,25-dihydroxycholecalciferol receptors in long bones of newborn rats.
作者信息
Sömjen D, Sömjen G J, Weisman Y, Binderman I
出版信息
Biochem J. 1982 Apr 15;204(1):31-6. doi: 10.1042/bj2040031.
Several reports have appeared that suggest that 24,25-dihydroxycholecalciferol has a possible biological role in bone formation. We have utilized competition studies, saturation analysis, sucrose-density-gradient sedimentation and DEAE-cellulose chromatography to demonstrate that long bones of vitamin D-depleted newborn rats contain cytoplasmic and possibly nuclear receptors that bind 24,25-dihydroxycholecalciferol with specificity and high affinity (Kd = 1.79 nM). Sucrose-density-gradient analysis of the cytoplasmic 24,25-dihydroxycholecalciferol-binding component showed a single binding macromolecule for 24,25-dihydroxycholecalciferol with a sedimentation coefficient of 3.1 S. DEAE-cellulose chromatography showed a [3H]24,25, dihydroxycholecalciferol-macromolecular complex that binds to DEAE-cellulose and elutes between 0.15 and 0.21 M-KCl. The finding of 24,25-dihydroxycholecalciferol receptors in long bones of newborn rats suggests a possible involvement of 24,25-dihydroxycholecalciferol in the metabolism of developing skeletal tissues.
已有数篇报道表明，24,25 - 二羟胆钙化醇在骨形成过程中可能具有生物学作用。我们运用竞争研究、饱和分析、蔗糖密度梯度沉降及二乙氨基乙基纤维素色谱法，证实维生素D缺乏的新生大鼠的长骨含有细胞质受体，可能还有核受体，这些受体能特异性且高亲和力地结合24,25 - 二羟胆钙化醇（解离常数Kd = 1.79 nM）。对细胞质中24,25 - 二羟胆钙化醇结合成分进行蔗糖密度梯度分析，结果显示存在一种沉降系数为3.1 S的单一结合大分子。二乙氨基乙基纤维素色谱法显示出一种[3H]24,25 - 二羟胆钙化醇 - 大分子复合物，该复合物可与二乙氨基乙基纤维素结合，并在0.15至0.21 M - KCl浓度范围内洗脱。在新生大鼠长骨中发现24,25 - 二羟胆钙化醇受体，提示24,25 - 二羟胆钙化醇可能参与发育中骨骼组织的代谢。
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