Helander H F, Durbin R P
Am J Physiol. 1982 Oct;243(4):G297-303. doi: 10.1152/ajpgi.1982.243.4.G297.
Autoradiography at the light microscopic level was used to localize sites of [3H]ouabain binding in isolated stimulated frog gastric mucosa. Silver grains denoting binding were located near basal and lateral surfaces in both oxyntic and surface epithelial cells. Binding to oxyntic cells occurred well before inhibition of acid secretion, consistent with the view that inhibition by this drug is indirect, and subsequent to inactivation of oxyntic cell Na+-K+-ATPase and the resulting depression of cellular K+. The presence of Na+-K+-ATPase in surface cells, as shown by binding of labeled ouabain, suggests a role for that transport enzyme in electrical activity and secretion of alkali by these cells.
利用光学显微镜水平的放射自显影技术，在分离的受刺激青蛙胃黏膜中定位[3H]哇巴因结合位点。表示结合的银颗粒位于壁细胞和表面上皮细胞的基底和侧面附近。壁细胞的结合早在酸分泌受到抑制之前就已发生，这与该药物的抑制作用是间接的观点一致，并且是在壁细胞钠钾ATP酶失活以及随后细胞内钾离子浓度降低之后发生的。标记的哇巴因结合显示表面细胞中存在钠钾ATP酶，这表明该转运酶在这些细胞的电活动和碱分泌中发挥作用。
