Balint J, Nagai T, Ikeda Y, Meek K, Terman D S
Clin Exp Immunol. 1982 Aug;49(2):433-40.
Sera from dogs with mammary adenocarcinoma were assessed for the presence of immune complexes (IC) and the physicochemical composition of these complexes was investigated. Employing 125I-anti-canine IgG as indicator, elevated levels of C1q binding IgG were detected in sera of dogs with mammary adenocarcinoma. Polyacrylamide gel electrophoresis (PAGE) analysis of IC isolated by G-200 fractionation and protein A affinity chromatography revealed the presence of a dense polypeptide band corresponding to the alpha chain of IgA which was present in the mammary adenocarcinoma sera but not in normal dog sera or sera from dogs with other tumours. Employing monospecific radiolabelled anti-canine IgA as indicator in solid phase C1q binding radioimmunoassays, significantly elevated levels of C1q binding IgA were detected in five of eight mammary adenocarcinoma sera but not in sera of normal dogs or other tumour bearing dogs (P less than 0.05). Sera from mammary adenocarcinoma bearing dogs treated with 5% polyethylene glycol (PEG) and subjected to sucrose density gradient ultracentrifugation revealed IgA containing IC in fractions greater than 7S to greater than 19S. Findings suggest that IC are present in sera of dogs with mammary adenocarcinoma and that that IgA is a major and unique component of these complexes and, hence, may play a significant role in the development and evolution of the canine immune response to mammary adenocarcinoma.
对患有乳腺腺癌的犬只血清进行免疫复合物（IC）检测，并研究这些复合物的物理化学组成。以125I-抗犬IgG作为指示剂，在患有乳腺腺癌的犬只血清中检测到C1q结合IgG水平升高。通过G-200分级分离和蛋白A亲和层析分离出的IC进行聚丙烯酰胺凝胶电泳（PAGE）分析，结果显示在乳腺腺癌血清中存在一条与IgAα链相对应的致密多肽带，而在正常犬血清或患有其他肿瘤的犬血清中则不存在。在固相C1q结合放射免疫分析中，以单特异性放射性标记抗犬IgA作为指示剂，在8份乳腺腺癌血清中的5份中检测到C1q结合IgA水平显著升高，而在正常犬血清或其他患肿瘤犬血清中未检测到（P<0.05）。对用5%聚乙二醇（PEG）处理并进行蔗糖密度梯度超速离心的患有乳腺腺癌犬只的血清进行分析，发现在大于7S至大于19S的组分中存在含IgA的IC。研究结果表明，IC存在于患有乳腺腺癌的犬只血清中，且IgA是这些复合物的主要且独特的成分，因此可能在犬对乳腺腺癌的免疫反应的发生和发展中发挥重要作用。
