Dráber P
Int J Immunopharmacol. 1982;4(5):401-5. doi: 10.1016/0192-0561(82)90013-3.
Concanavalin A-induced activation of thymus cells compared to spleen or lymph node activation is extremely sensitive to the inhibitory effect of hydrocortisone (HC). This inhibitory effect of thymus cells, as observed at concentrations of 10(-7) M and higher, can be abrogated (at concentrations of up to 10(-5) M HC) by the addition of culture supernatants containing the product of activated T cells--Interleukin 2 (IL2), but not the supernatants containing the product of activated macrophages--Interleukin 1 (IL1). The protective effect of the IL2-containing supernatants can in part be removed by absorption with T cell blasts, but not B cell blasts. These results support the following notion of the mechanism of T cell activation. IL1 induces IL2 production by helper T cells and IL2, in turn, initiates proliferation of the sensitive T cells with IL2 receptors. These observations suggest that the inhibitory effect of HC on thymus cell activation results from inhibition of IL2 production.
与脾脏或淋巴结激活相比，伴刀豆球蛋白A诱导的胸腺细胞激活对氢化可的松（HC）的抑制作用极为敏感。在浓度为10⁻⁷M及更高时观察到的胸腺细胞的这种抑制作用，可通过添加含有活化T细胞产物——白细胞介素2（IL2）的培养上清液（在HC浓度高达10⁻⁵M时）来消除，但不能通过添加含有活化巨噬细胞产物——白细胞介素1（IL1）的上清液来消除。含IL2的上清液的保护作用部分可通过用T细胞母细胞吸收而去除，但不能通过B细胞母细胞吸收来去除。这些结果支持了以下关于T细胞激活机制的观点。IL1诱导辅助性T细胞产生IL2，而IL2反过来启动具有IL2受体的敏感T细胞的增殖。这些观察结果表明，HC对胸腺细胞激活的抑制作用是由于抑制了IL2的产生。
