Stern N, Tietz A
Biochim Biophys Acta. 1978 Sep 28;530(3):357-66. doi: 10.1016/0005-2760(78)90155-8.
A 247 000 x g particulate fraction from a moderately halophilic halotolerant bacterium incorporated [14C] glucose added as UDP[14C]glucose and 32P-labeled phosphatidylglycerol into glucosylphosphatidylglycerol. Exogenously added phosphatidylglycerol was available to the enzyme only when dispersed in a detergent, preferably Triton X-405, by sonication. The 14C- or 32P-labeled glucosylphosphatidylglycerol was degraded with phospholipase C. The water soluble product formed was isolated and identified by paper chromatography as glucosylglycerolphosphate. The system required Mg2+ or Ca2+ for activity. KCl and NaCl were inhibitory even when added at low concentrations.
来自中度嗜盐耐盐细菌的247000 x g微粒部分将添加的[14C]葡萄糖（以UDP[14C]葡萄糖形式）和32P标记的磷脂酰甘油掺入葡糖基磷脂酰甘油中。只有当通过超声处理分散在去污剂（最好是 Triton X-405）中时，外源添加的磷脂酰甘油才能被该酶利用。用磷脂酶C降解14C或32P标记的葡糖基磷脂酰甘油。通过纸色谱法分离并鉴定形成的水溶性产物为葡糖基甘油磷酸。该系统需要Mg2+或Ca2+来发挥活性。即使以低浓度添加，KCl和NaCl也具有抑制作用。
