[普鲁卡因系列局部麻醉药生理浓度对脑突触体膜结构和功能状态的影响]

[Effect of physiologic concentrations of local anesthetics of the procaine series on the structural and functional state of cerebral synaptosome membranes].

作者信息

Aksentsev S L, Okun' I M, Rakovich A A, Miliutin A A, Konev S V

出版信息

Biofizika. 1978 Sep-Oct;23(5):821-6.

Abstract

Studies are presented of the effect of procaine group anesthetics on rat brain synaptosome stability to dodecyl sulfate and on catalytic properties of the membrane bound alkaline phosphatase. The dose curves of detergent stability are characterized by two maxima, one at 3.10(-4) M for tetracaine, 2.10(-6) M for lidocaine and 5.10(-5) M for procaine; the other being at 10(-3) M for all anesthetics. The curves of Vmax and KM versus procaine concentration to exhibit the minimum at 5.10(-7) M and maximum at 3,2.10(-6) M. Procaine at 5.10(-7) M increases enthalpy and enthropy of membraneous alkaline phosphatase. It is suggested that interactions between anesthetics and centers of high affinity lead to synaptosome structural rearrangements, which affect the properties of membraneous enzymes.

摘要

本文介绍了普鲁卡因类麻醉剂对大鼠脑突触体对十二烷基硫酸钠的稳定性以及膜结合碱性磷酸酶催化特性的影响。去污剂稳定性的剂量曲线具有两个最大值，一个是丁卡因在3×10⁻⁴ M时，利多卡因在2×10⁻⁶ M时，普鲁卡因在5×10⁻⁵ M时；另一个是所有麻醉剂在10⁻³ M时。Vmax和KM与普鲁卡因浓度的曲线在5×10⁻⁷ M时显示最小值，在3×2×10⁻⁶ M时显示最大值。5×10⁻⁷ M的普鲁卡因增加了膜碱性磷酸酶的焓和熵。有人认为麻醉剂与高亲和力中心之间的相互作用会导致突触体结构重排，从而影响膜酶的特性。