通过T细胞克隆检测人类外周血中存在的对6-硫鸟嘌呤耐药的突变淋巴细胞。
T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood.
作者信息
Albertini R J, Castle K L, Borcherding W R
出版信息
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6617-21. doi: 10.1073/pnas.79.21.6617.
Abstract
Rare thioguanine-resistant T lymphocytes, present in vivo in human peripheral blood, were isolated and grown in vitro as thioguanine-resistant cultured T cells. The conditions for their selection in vitro were such that thioguanine resistance had to have arisen in vivo. The mutant cells bore T-cell surface markers, maintained their thioguanine resistance in vitro in the presence or absence of selection, and were deficient in hypoxanthine-guanine phosphoribosyltransferase activity.
摘要
在人外周血中体内存在的罕见硫鸟嘌呤抗性T淋巴细胞被分离出来，并作为硫鸟嘌呤抗性培养T细胞在体外培养。它们在体外的选择条件使得硫鸟嘌呤抗性必定已在体内产生。突变细胞带有T细胞表面标志物，在有或没有选择的情况下在体外维持其硫鸟嘌呤抗性，并且次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶活性缺乏。
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